Exhibit 21.1
Subsidiaries of the Registrant
(As of December 31, 2025)
| Subsidiary Name | Jurisdiction of Formation | |
| 1. Guardian Pharmacy of Arizona, LLC | Indiana | |
| 2. Guardian Pharmacy of Atlanta, LLC | Georgia | |
| 3. Guardian Pharmacy of Birmingham, LLC | Georgia | |
| 4. Guardian Pharmacy of Boise, LLC | Georgia | |
| 5. Guardian Pharmacy of Cincinnati, LLC | Georgia | |
| 6. Guardian Pharmacy of Colorado, LLC | Georgia | |
| 7. Guardian Pharmacy of Columbus, LLC | Georgia | |
| 8. Guardian Pharmacy of Daytona, LLC | Georgia | |
| 9. Guardian Pharmacy of Denver, LLC | Georgia | |
| 10. Guardian Pharmacy of Eastern NC, LLC | Georgia | |
| 11. Guardian Pharmacy of Grand Island, LLC | Georgia | |
| 12. Guardian Pharmacy of Grand Rapids, LLC | Georgia | |
| 13. Guardian Pharmacy of Idaho Falls, LLC | Georgia | |
| 14. Guardian Pharmacy of Indianapolis LTC, LLC | Georgia | |
| 15. Guardian Pharmacy of Indianapolis Nuclear, LLC | Georgia | |
| 16. Guardian Pharmacy of Iowa, LLC | Georgia | |
| 17. Guardian Pharmacy of Jacksonville, LLC | Georgia | |
| 18. Guardian Pharmacy of Kansas City, LLC | Georgia | |
| 19. Guardian Pharmacy of Kentucky, LLC | Georgia | |
| 20. Guardian Pharmacy of Knoxville, LLC | Georgia | |
| 21. Guardian Pharmacy of Madison, LLC | Georgia | |
| 22. Guardian Pharmacy of Minneapolis, LLC | Georgia | |
| 23. Guardian Pharmacy of Maine, LLC | Georgia | |
| 24. Guardian Pharmacy of Minnesota, LLC | Georgia | |
| 25. Guardian Pharmacy of Missouri, LLC | Georgia | |
| 26. Guardian Pharmacy of Montana, LLC | Georgia | |
| 27. Guardian Pharmacy of New Jersey, LLC | Georgia | |
| 28. Guardian Pharmacy of Northern Virginia, LLC | Georgia | |
| 29. Guardian Pharmacy of NW Florida, LLC | Georgia | |
| 30. Guardian Pharmacy of Oklahoma, LLC | Georgia | |
| 31. Guardian Pharmacy of Oklahoma City, LLC | Georgia | |
| 32. Guardian Pharmacy of Omaha, LLC | Georgia | |
| 33. Guardian Pharmacy of Oregon, LLC | Georgia | |
| 34. Guardian Pharmacy of Orlando, LLC | Georgia | |
| 35. Guardian Pharmacy of Piedmont Carolinas, LLC | Georgia | |
| 36. Guardian Pharmacy of South Carolina One, LLC | Georgia | |
| 37. Guardian Pharmacy of Southeast Florida, LLC | Georgia | |
| 38. Guardian Pharmacy of Southeast Georgia, LLC | Georgia | |
| 39. Guardian Pharmacy of Southern California, LLC | Georgia | |
| 40. Guardian Pharmacy of Southwest Florida, LLC | Georgia | |
| 41. Guardian Pharmacy of St. Louis, LLC | Georgia | |
| 42. Guardian Pharmacy of Tampa, LLC | Georgia | |
| 43. Guardian Pharmacy of Tennessee One, LLC | Georgia | |
| 44. Guardian Pharmacy of Tennessee Two, LLC | Georgia | |
| 45. Guardian Pharmacy of Tennessee Three, LLC | Georgia | |
| 46. Guardian Pharmacy of Texas, LLC | Georgia | |
| 47. Guardian Pharmacy of Tucson, LLC | Georgia | |
| 48. Guardian Pharmacy of Tulsa, LLC | Georgia | |
| 49. Guardian Pharmacy of Utah, LLC | Georgia | |
| 50. Guardian Pharmacy of Virginia, LLC | Georgia | |
| 51. Guardian Pharmacy of Washington, LLC | Georgia | |
| 52. Guardian Pharmacy of Wichita, LLC | Georgia | |
| 53. Guardian Pharmacy Services Management, LLC | Georgia | |
| 54. Guardian Pharmacy, LLC | Indiana |